Release of HBP, elastase, cathepsin G, and proteinase 3 from purified neutrophils.

Neutrophils were incubated in the absence (■) or presence of fMLP (100 nM) and cytochalasin B (10 μM) (⋄) for 30 minutes at 37°C. Cells were centrifuged, and 50 μL supernatant was applied in serial dilutions (2ⁿ) to microtiter plates. This was followed by detection with antibodies to HBP (A), elastase (B), cathepsin G (C), and proteinase 3 (D). Results are the mean ± SD of 3 independently performed experiments.