Macroscopic observations of the PDGF-AA and PGDF-BB effect on bFGF-induced neovascularization in the CAM assay.

Gelatin sponge adsorbed with bFGF (500 μg) is surrounded by allantoic vessels that develop radially toward the implant in a spoked-wheel pattern (A), whereas no vascular reaction is detectable around PBS-treated (B) and PDGF-AA–treated (C) sponges. Allantoic vessels were less numerous in the specimens treated with bFGF added to PDGF-AA and PDGF-BB (500 μg each) (D and F, respectively), whereas PDGF-BB alone induced a moderate angiogenic response (E). Asterisks indicate sponges. Panels refer to a representative experiment. Original magnification, × 50.