FL treatment does not change allostimulatory functions of CD8α+ and CD8α− DC subclasses.
(A) Allogeneic B6 T cells were cultured with various numbers of each DC subset isolated from FL- and control-treated mice. T-cell responses to CD8α+ (open circle) and CD8α− (open square) DCs from control-treated mice and responses to CD8α+ (closed circle) and CD8α− (closed square) DCs from control-treated mice were determined after 3-day MLR by 3H-thymidine incorporation. Data shown are the mean ± SD of quadruplicate cultures. (B-D) Sorted CD8α+ and CD8α− fractions from control DCs (open bar) or FL DCs (closed bar) were mixed at various ratios and cultured with allogeneic T cells from B6 mice for 3 days. Cells were then pulsed with3H-thymidine for the last 16 hours of culture, and proliferation was determined (B). Supernatant was collected 48 hours after culture for IFN-γ measurement (C) and IL-2 measurement (D) by ELISA.
