Impaired allostimulatory activity of FL DCs in MLR.
(A) Five thousand irradiated DCs isolated from B6D2F1 mice were cultured with 105 T cells from either B6 (allogeneic) or B6D2F1 (syngeneic) mice as described in “Materials and methods.” T-cell proliferation was determined by 3H-thymidine incorporation. Allogeneic T-cell responses to control DCs (open circle) and to FL DCs (closed circle) and syngeneic T-cell responses to control DCs (open square) and to FL DCs (closed square) are shown. Data are the mean ± SD of quadruplicate cultures. (B, C) Supernatants of cultures with control DCs (open bar) or with FL DCs (closed bar) were collected at 24 hours for IL-2 (B) and at 72 hours for IFN-γ (C) and measured by ELISA as described in “Materials and methods.”
