Expression of NKp46 in freshly derived NK cells from TAP2^−/− patients.

PBMC from the patients and from a healthy control donor were depleted of CD3⁺, HLA-DR⁺, and adherent cells and were analyzed by double fluorescence for the expression of NKp46, CD56, and CD16. This analysis led to the identification of different NK cell subsets. Histograms on the left show the percentages of the CD56⁺CD16⁺NKp46^bright cells (black bars), CD56^brightCD16⁻NKp46^brightcells (gray bars), and CD56⁺CD16⁺NKp46^dull cells (white bars) in the 2 patients and in the healthy donor. Cytofluorometric profiles on the right show the overall surface expression of NKp46 in the same donors.