Phenotypes of peripheral B cells of CVID patients.

PBMCs were stained for the expression of CD19, CD27, IgM, and IgD and analyzed by FACS. Analysis was performed by gating on CD19⁺cells, and dead cells were excluded by forward/side scatter gating. Representative examples of each CVID subgroup (A-C) according to the classification by Bryant et al9 as well as one healthy control (HD) are given. Indicated values represent percentages of gated CD19⁺ B cells. See “Patients, materials, and methods.”