Detection of

ETV6-ACS2 fusion transcripts in the aCML case with a t(5;12)(q31;p13) (case 7). (Ai) RT-PCR confirmed the fusion of ETV6 to ACS2, as identified by 3′-RACE in the t(5;12) case (case 7). (Aii) Hybridization with an internal oligonucleotide revealed the existence of 3 different fusion transcripts, which was confirmed by cloning and sequencing of these RT-PCR products, shown in the lower panel. (B) Representation of the identified fusion transcripts. Open reading frames were determined on the basis of the start codon of ETV6. Only transcript C is an in-frame fusion. Exon numbering of ACS2 is according to Yagasaki et al.22