American Society of Hematology

Fig. 5.

$Fig. 5. Failure of C2-ceramide to inhibit ARF1 or RhoA association with the plasma membrane. / PMNs were treated with C2-ceramide and stimulated as indicated for Figure 4. Cells were probe sonicated and fractionated on 15% to 35% discontinuous sucrose gradients as described in “Materials and methods” to isolate plasma membrane. Equal amounts of protein were run on 15% SDS-PAGE, and Western blots were probed with antibody against ARF1 or RhoA. U indicates unstimulated; CB, cytochalasin B; F, FMLP, and C2, C2-ceramide. One representative experiment of 3.$

Failure of C₂-ceramide to inhibit ARF1 or RhoA association with the plasma membrane.

PMNs were treated with C₂-ceramide and stimulated as indicated for Figure 4. Cells were probe sonicated and fractionated on 15% to 35% discontinuous sucrose gradients as described in “Materials and methods” to isolate plasma membrane. Equal amounts of protein were run on 15% SDS-PAGE, and Western blots were probed with antibody against ARF1 or RhoA. U indicates unstimulated; CB, cytochalasin B; F, FMLP, and C2, C₂-ceramide. One representative experiment of 3.

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