Nucleotide sequence analyses.

(A) Nucleotide sequence analysis of amplified GPIIIa cDNA derived from an Oe^a (−) individual (HPA-1b/1b) and the Oe^a (+) (HPA-1b/1b) grandfather. PCR products encompassing bases 56-698 (left panel) and bases 1666-2415 (right panel) were subcloned into the plasmid vector pGEM-5Zf and sequenced. The missing of the triplet AAG (underlined) in Oe^a (+) individual results in ΔLys₆₁₁. Both individuals carry the Pro₃₃ GPIIIa form, which corresponds to their HPA-1b phenotypes. (B) Nucleotide sequence analysis of amplified GPIIIa DNA derived from Oe^a (+) and Oe^a (−) individuals. PCR products encompassing the entire exon 10 were sequenced directly. The mutated GPIIIa allele of Oe^a (+) individual is shown by the asterisks (top panel). The deletion of the triplet AAG (underlined) resulting in a shift of 3 bases was observed in Oe^a (+), but not in Oe^a (−) individual (bottom panel).