Fig. 4.
Fig. 4. Immunoprecipitation analysis of chymotrypsin-treated platelets. / Paternal platelets (Oea [+], HPA-1a/1b, HPA-4a/4a) were surface labeled with biotin, digested with chymotrypsin, lysed, and precipitated with anti–HPA-1a (lane 1), anti-Oea (lane 2), anti-HPA-4a (lane 3), and normal human serum (lane 4). Immunoprecipitates were analyzed on 7.5% SDS-PAGE under nonreducing conditions, blotted, and visualized with chemiluminescence substrate.

Immunoprecipitation analysis of chymotrypsin-treated platelets.

Paternal platelets (Oea [+], HPA-1a/1b, HPA-4a/4a) were surface labeled with biotin, digested with chymotrypsin, lysed, and precipitated with anti–HPA-1a (lane 1), anti-Oea (lane 2), anti-HPA-4a (lane 3), and normal human serum (lane 4). Immunoprecipitates were analyzed on 7.5% SDS-PAGE under nonreducing conditions, blotted, and visualized with chemiluminescence substrate.

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