Fig. 3.
Fig. 3. Immunoblot of Oea (+) platelets. / Paternal platelet lysate was separated on 7.5% SDS-PAGE under nonreducing conditions and transferred onto nitrocellulose paper. After incubation with eluates from normal human serum (lane 1), anti-Oea (lane 2), and anti–HPA-1a (lane 3) bound IgG were detected with BCIP as substrate; ns indicates nonspecific binding.

Immunoblot of Oea (+) platelets.

Paternal platelet lysate was separated on 7.5% SDS-PAGE under nonreducing conditions and transferred onto nitrocellulose paper. After incubation with eluates from normal human serum (lane 1), anti-Oea (lane 2), and anti–HPA-1a (lane 3) bound IgG were detected with BCIP as substrate; ns indicates nonspecific binding.

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