Detection of MMP-9 mRNA in leukemic cells obtained from patients with ATL by RT-PCR analysis.

(A) Lanes 1 and 2, RNA from normal resting PBMCs; lane 3, RNA from PHA blasts; lanes 4-11, RNA from PBMCs from patients with ATL. (A) indicates acute type; (C), chronic type. Bottom, ethidium bromide–stained gel showing β-actin PCR products. Arrows indicate positions of the specifically amplified DNA. (B) Quantitative hybridization capture assay to detect amplified nucleic acid of MMPs. Results show relative levels of the MMPs/β-actin compared with MMPs/ β-actin in normal 1.