Fig. 3.
Induction kinetics of the MMP-9 gene in JPX-9 cells treated with CdCl2.
(A) Total RNA samples were prepared from CdCl2-treated JPX-9 cells at the indicated time points (0-72 hours). The expression of Tax and MMPs in the extracted RNA was analyzed by Northern blot and RT-PCR analysis, respectively. GAPDH served as a loading control for Northern analysis, whereas β-actin served as an internal control in the RT-PCR procedure. (B) Quantitative hybridization capture assay to detect amplified nucleic acid of MMPs. Results are shown quantitatively as fold increase over the amount at time zero.
