Zymographic analysis of conditioned media from various T-cell lines.

(A) A total of 12 μL conditioned medium was subjected to electrophoresis on 10% SDS-polyacrylamide gels containing 1 mg/mL gelatin. SDS was removed following electrophoresis, and gels were incubated to restore gelatinase activity. Lane 1, conditioned media from PBMCs activated by PMA (positive control for MMP-2 and MMP-9). Major gelatinolytic activities corresponding to pro–MMP-9 (92 kd) and pro–MMP-2 (68 kd) are indicated. (B) Densitometric scan of MMP bands in (A) shows relative levels of the amounts of MMPs in each line compared with those in HTLV-I–negative control MOLT-4 cells.