Northern blot analysis of HTLV-I mRNA expression and RT-PCR analysis of MMP-2 and MMP-9 mRNA levels in various HTLV-I–infected and uninfected human T-cell lines.

(A) Total RNA was prepared from the indicated T-cell lines. Predominant HTLV-I mRNA species of 2.1, 4.2, and 8.5 kb were detected in HPB-ATL-2, HPB-CTL-I, HLN-ATL-O, and HUT-102 cell lines (lanes 4-7). GAPDH and β-actin expression served as controls. (B) Quantitative hybridization capture assay to detect amplified nucleic acid of MMPs. Expression levels of MMP mRNA were calculated from the ratios of the amount of amplification product of MMPs to that of β-actin. Results show relative levels of the MMPs/β-actin in each line compared with that in HTLV-I–negative Jurkat cells.