ATP-treated mature DCs attract Th1 and Tc1 clones less efficiently but not Th2 or Tc2 clones.

Supernatants from untreated DCs (○) or DCs stimulated with either 250 μM ATP (■), 250 μM UTP (▵), 10 μg/mL LPS (●), LPS and ATP (▪), or LPS and UTP (▴) were diluted with 0.5% BSA RPMI to stimulate resting type 1 or type 2 T-cell clones in a migration assay. Data are mean ± SD net migration results from 3 different T-cell clones of each type. Differences in T-cell migration induced by supernatants from immature (A,C,E,G) compared with mature (B,D,F,H) DCs, as well as differences between DCs stimulated with LPS alone and DCs stimulated with LPS and ATP (B,F), were significant (P < .03) at all supernatant dilutions.