ATP inhibits IP-10 and RANTES release and augments MDC secretion in a dose-dependent fashion.

Immature DCs were stimulated with 10 μg/mL LPS and a graded concentration of ATP (▪) or UTP (▴). After 24 hours of incubation, IP-10 (A), RANTES (B), MDC (C), and TARC (D) were measured in culture supernatants by ELISA. Results are mean ± SD nanograms per milliliter/10⁶ cells from triplicate cultures. Differences in IP-10, RANTES, and MDC secretion of DCs stimulated with LPS alone and those stimulated with both LPS and ATP were significant (P < .05) when ATP amounts were at least 2.5 μM, at least 100 μM, and at least 2.5 μM, respectively, for the 3 chemokine assessments.