Functional expression of CXCR4, CCR7, and CCR5 in immature or maturing DCs exposed to ATP.

DCs were incubated for 24 hours with ATP alone, LPS alone, LPS and ATP, or none of these agents; loaded with 8 μM fluo-3–acetoxymethyl ester in the presence of 1 μM pluronic F-127; and tested for intracellular calcium mobilization in response to SDF-1α (CXCR4 ligand), MIP-3β (CCR7 ligand), and MIP-1β (CCR5 ligand) (all at a concentration of 100 ng/mL). Calcium flux was measured with a FACScan device. Results are the kinetics of MFI and are representative of 4 independent experiments.