Fig. 3.
Fig. 3. Simultaneous detection of the. / NUP98-HOXA9 and NUP98-HOXA13 orNUP98-HOXA11 transcripts in patients S and Y. (A) The 378-bp NUP98-HOXA9 products were detected in patients S, Y, and J, the patient with t(7;11) AML with a HOXA9break. However, NUP98-HOXA7 and NUP98-HOXA10chimeras were not detected. In addition, the NUP98-HOXA11chimera was not observed in patient S. ddw indicates water control. (B) Structures of fusion transcripts and predicted proteins ofNUP98-HOXA13, NUP98-HOXA11, andNUP98-HOXA9 of the current patients with leukemia. (C) LD-PCR showed NUP98-HOXA fusion genes. Reverse primers for each HOX gene were indicated at the top, and the sample identity was indicated at the middle. Serial dilutions of J sample by patient S were also subjected to long-distance-PCR to check the detection limit of the NUP98-HOXA9 fusion. M indicates λ/HindIII DNA size marker.

Simultaneous detection of the

NUP98-HOXA9 and NUP98-HOXA13 orNUP98-HOXA11 transcripts in patients S and Y. (A) The 378-bp NUP98-HOXA9 products were detected in patients S, Y, and J, the patient with t(7;11) AML with a HOXA9break. However, NUP98-HOXA7 and NUP98-HOXA10chimeras were not detected. In addition, the NUP98-HOXA11chimera was not observed in patient S. ddw indicates water control. (B) Structures of fusion transcripts and predicted proteins ofNUP98-HOXA13, NUP98-HOXA11, andNUP98-HOXA9 of the current patients with leukemia. (C) LD-PCR showed NUP98-HOXA fusion genes. Reverse primers for each HOX gene were indicated at the top, and the sample identity was indicated at the middle. Serial dilutions of J sample by patient S were also subjected to long-distance-PCR to check the detection limit of the NUP98-HOXA9 fusion. M indicates λ/HindIII DNA size marker.

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