Fig. 7.
SOCS-2 expression inBCR-ABL–positive andBCR-ABL–negative cell lines after exposure to inhibitors of signal transduction pathways.
We exposed 3-5 × 105 cells to 25 μM LY294002 for 1 hour, 10 μM U0126 for 1 hour, 10 μM manumycin A for 2.5 to 4 hours, or 10 μM AG490 for 24 hours. SOCS-2 expression was determined by real-time RT-PCR and normalized for GAPDH levels. Results represent the SOCS-2 levels as compared to nonexposed control cells. BCR-ABL–positive, gray bars;BCR-ABL–negative, black bars.
