Activation of alveolar macrophages by GM-CSF transgene expression.

Mice were treated as in as the intranasal group in Figure 5B. Macrophages were purified by adherence and cultured without or with LPS (1.2 μg/mL) for 3 days, and supernatants were measured for NO by a biochemical method. Results are expressed as a mean ± SEM from triplicate wells and are representative of 2 independent experiments. The difference between CP/Addl70.3 and CP/Ad/GM-CSF is statistically significant (P = .0013).