Unimpaired TNF-α and NO responses in alveolar macrophages in CP-treated mice.

Macrophages were obtained from PBS-treated control mice or mice that had 5 consecutive treatments of CP (day 10 after the first CP) and were cultured for 3 days without or with stimulation by IFN-γ (800 pg/mL), LPS (1.2 μg/mL), or IFN-γ plus LPS (same concentrations). Supernatants were measured by ELISA for TNF-α (A) and by a biochemical method for NO (B). Results are expressed as mean ± SEM from 3 independent experiments (triplicate wells per condition in each experiment).