Effect of Myb ODN purging on brc/abl–expressing primitive hematopoietic stem/progenitor cells.

Myb ODN purging preferentially affects malignant (brc/abl–expressing) primitive hematopoietic stem/progenitor cells. (A) Ethidium bromide–stained agarose gel containing c-myb, bcr-abl, and β-actin mRNA RT-PCR products derived from patient no. 16's CD34⁺cells before marrow purging (b/p); whole bone marrow cells (BM) before purging; CD34⁺ cells after purging (a/p); LTCICs; and water (H₂O) after exposure to the c-myb–targeted ODN for 72 hours. Lanes containing molecular weight markers are indicated by the symbol Ψ. (B) Ethidium bromide–stained agarose gel demonstrating β-actin (lanes 1-3), c-myb (lanes 4-6), and bcr/abl mRNA (lanes 7-9) expression in CFU-GM colonies derived from a single patient's untreated (lanes 1, 4, 7) or anti-Myb ODN–purged (lanes 2, 5, 8) LTCICs. Respective H₂O control reactions are shown in lanes 3, 6, and 9. Molecular weight markers: Ψ.