Overexpression of

HOX-A10 slightly reduces the generation of CD15⁺ granulocytic cells and blocks their final maturation. (A) Four-color flow cytometric analysis of cells recovered from MS-5 stromal cell cultures in the presence of SCF, FL, TPO, and G-CSF, initiated with IRES-EGFP– and HOX-A10-IRES-EGFP–transduced CD34⁺ Lin⁻ CB cells. Dot plots show FSC compared with EGFP and HLA-DR-APC compared with CD15-PE for IRES-EGFP– and HOX-A10-IRES-EGFP–transduced CD34⁺ Lin⁻ CB cells on day 14 of culture. Quadrants were set to include at least 99% of cells stained with isotype controls in the lower left quadrants. Values in the corresponding dot plots indicate the percentage of cells in the corresponding quadrant. (B) Wright-Giemsa staining of EGFP⁺CD15⁺ cells sorted from IRES-EGFP–transduced cell cultures shown in panel A. The arrow indicates mature granulocytes. (C) Wright-Giemsa staining of EGFP⁺ CD15⁺ cells sorted from HOX-A10-IRES-EGFP–transduced cell cultures shown in panel A. Original magnification B-C, × 200.