HOX-A10 –transduced monocytes can generate dendritic cells.

(A) Histograms show CD14 staining of sorted EGFP⁺CD14⁺ monocytes generated from IRES-EGFP– and HOX-A10-IRES-EGFP–transduced CD34⁺ Lin⁻ CB cells after 9 days of culture in MS-5 stromal cells in the presence of SCF, FL, TPO, and GM-CSF. (B) Flow cytometric analysis of cells generated from sorted IRES-EGFP– and HOX-A10-IRES-EGFP–transduced CD14⁺ cells shown in panel A after 4 days of culture in the presence of GM-CSF, TNF-α, and IL-4. Histograms show CD14 staining, and bivariate dot plots show CD80-PE compared with HLA-DR-APC. Values indicate the percentage of cells in the corresponding quadrant.