Overexpression of

HOX-A10 enhances differentiation of human monocytes. (A) EGFP-expression profile of IRES-EGFP– and HOX-A10-IRES-EGFP–transduced CD34⁺ Lin⁻ CB cells at initiation (day 0) and harvesting (day 14) of MS-5 stromal cell cultures in the presence of SCF, FL, TPO, and GM-CSF. Upper dot plots show FSC compared with EGFP. Values in the corresponding dot plots indicate the percentage of EGFP⁺ cells. Lower dot plots show 4-color flow cytometric analysis of the MS-5 stromal cell cultures on day 14, gated on EGFP⁺ cells. Dot plots show CD4-APC compared with CD14-PE and HLA-DR-APC compared with CD14-PE. Quadrants were set to include at least 99% of cells stained with isotype controls in the lower left quadrants. Values indicate the percentage of cells in the corresponding quadrants. Panels B and C show Wright-Giemsa staining of EGFP⁺ CD14⁺ cells sorted from IRES-EGFP–transduced cell cultures (B) or HOX-A10-IRES-EGFP–transduced cell cultures (C). Arrows indicates monocytes (m) or macrophages (mφ). Original magnification B-C, × 200.