Reduced NK-cell and B-cell differentiation potential of

HOX-A10–transduced CB stem cells.EGFP-expression profile of IRES-EGFP– and HOX-A10-IRES-EGFP–transduced CD34⁺ Lin⁻ CB cells at initiation (day 0) and harvesting (day 21) of MS-5 stromal cell cultures in the presence of either SCF, IL-2, and IL-15 or SCF and IL-7. Upper dot plots show FSC compared with EGFP. Values in the corresponding dot plots indicate the percentage of EGFP⁺cells. Lower dot plots show 4-color flow cytometric analysis of the MS-5 stromal cell cultures on day 21, gated on EGFP⁺ cells. Dot plots show CD56-APC compared with CD45-PE and HLA-DR-APC compared with CD19-PE. Quadrants were set to include at least 99% of cells stained with isotype controls in the lower left quadrants. Values in the corresponding dot plots indicate the percentage of cells in the upper right quadrant.