HOX-A10 –transduced CB progenitors have a strongly reduced T-cell differentiation potential in FTOC.

(A) Kinetic analysis of the percentage of EGFP-expressing cells in FTOC initiated with IRES-EGFP–transduced and HOX-A10-IRES-EGFP–transduced CD34⁺ Lin⁻ CB cells. Dot plots gated on human thymocytes show forward scatter (FSC) compared with EGFP for IRES-EGFP– (top) and HOX-A10-IRES-EGFP–transduced CD34⁺Lin⁻ CB cells (bottom) at the indicated time points of FTOC. Figures in the corresponding dot plots indicate the percentage of EGFP⁺ cells. (B) Bivariate dot plots (gated on human EGFP⁺ cells on day 25 of FTOC) of flow cytometric results with IRES-EGFP– (top) and HOX-A10-IRES-EGFP–transduced cells (bottom) showing CD4-APC compared with CD8β-PE expression and TCRαβ-PE or TCRγδ-PE compared with CD3-APC. Quadrants were set to include at least 99% of cells stained with isotype controls in the lower left quadrants. Values indicate the percentage of cells in the corresponding quadrants.