Flow cytometric analysis of normal BMMCs stained with anti-CD34 antibody, anti-Fas (CD95) antibody, and 7AAD.

(A) Scattergram of forward light scatter (FSC) versus right-angle light scatter (SSC), to allow gating on the BMMCs (low FSC and low SSC properties) (R1). (B) Scattergram of anti-CD34 fluorescence versus SSC gated on R1, to allow gating on CD34⁺ cells (R2). (C) Scattergram of FSC versus 7AAD fluorescence gated on R2, showing 7AAD^bright (dead), 7AAD^dim (apoptotic), and 7AAD⁻ (live) cells. (D) Scattergram of anti-CD34 versus anti-Fas fluorescence gated on R2, showing Fas⁺ and Fas⁻ CD34⁺ cells. Similar staining patterns were obtained for RA BM samples.