RT-PCR analysis for CCR4 and its ligands TARC/CCL17 and MDC/CCL22 in normal and ATL skin tissues.

Total RNA and DNA were prepared from normal skin tissues from 3 adult donors (2, 3, 4) and lesional skin tissues from 4 patients with ATL (5, 6, 7, 8). RT-PCR was carried out for CCR4, MDC, TARC, and G3PDH as described in “Materials and methods.” Genomic PCR was carried out for tax as described in “Materials and methods.” (1) No RNA. (2-4) Normal skin tissues. (5-8) Lesional skin tissues from patients with ATL.