Effect of ODC overexpression on type XVIII collagen and endostatin expression, as well as endothelial proliferation.

Both total RNA and whole cell lysates were prepared from ODC transfectants and mock transfectants, as well as DFMO-treated ODC transfectants. (A) Type XVIII collagen (COL XVIII), VEGF, and GAPDH mRNA expression was analyzed by RT-PCR. (B) Concentrated conditioned media from both ODC transfectants and mock transfectants were used for endostatin analysis by Western blotting. (C) The concentration of endostatin was quantitated by using an endostatin enzyme immunoassay kit. (D) Conditioned media derived from both ODC transfectants and mock transfectants were assayed for CPAE cell proliferation.