American Society of Hematology

Fig. 4.

$Fig. 4. Double-transduced K7 cells with wt dCK and deletion exon 5 dCK. / (A) Transduced cells were enriched by FACS sorting on the basis of truncated NGFR and GFP as marker genes. (B) Target gene expression was determined by RT-PCR analysis using human-specific dCK PCR primers. PCR fragments were separated on 1.5% agarose gels and visualized by ethidium bromide staining. (C) Dose-response curves were generated by WST-1 assays after 48 and 72 hours of AraC incubation. Metabolic activities of the cells in the presence of increasing concentrations of AraC (x-axis) were analyzed by the cell proliferation assay WST-1. Y-axis (fraction control) represents the metabolic activity of the cells in the presence of AraC divided by the metabolic activity of cells grown in the absence of AraC. (♦) untransduced K7 cells; (▴) K7/wt cells; and (×) K7/wt+del 5 double-transduced cells.$

Double-transduced K7 cells with wt dCK and deletion exon 5 dCK.

(A) Transduced cells were enriched by FACS sorting on the basis of truncated NGFR and GFP as marker genes. (B) Target gene expression was determined by RT-PCR analysis using human-specific dCK PCR primers. PCR fragments were separated on 1.5% agarose gels and visualized by ethidium bromide staining. (C) Dose-response curves were generated by WST-1 assays after 48 and 72 hours of AraC incubation. Metabolic activities of the cells in the presence of increasing concentrations of AraC (x-axis) were analyzed by the cell proliferation assay WST-1. Y-axis (fraction control) represents the metabolic activity of the cells in the presence of AraC divided by the metabolic activity of cells grown in the absence of AraC. (♦) untransduced K7 cells; (▴) K7/wt cells; and (×) K7/wt+del 5 double-transduced cells.

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