Target gene expression in retrovirally transduced cells.

Target gene expression after retroviral transfection was analyzed by RT-PCR amplifications using rat-specific (upper panel) or human-specific (lower panel) dCK PCR-primers. (A) KA cells. (B) K7 cells. Lane 1, parental cells; lane 2, cells transduced with empty vector controls; lane 3, cells transduced with human wt dCK; lane 4, cells transduced with deletion exon 5 human dCK; lane 5, cells transduced with deletion exons 3 to 4 human dCK; lane 6, cells transduced with deletion exons 3 to 6 dCK. PCR fragments were separated on a 1.5% agarose gel and visualized by ethidium bromide staining.