Frequency of LCMV-specific T cells and total spleen cell numbers in CCR5^−/− and wild-type (wt) mice after intravenous LCMV Traub infection.

CCR5^−/− and wt mice were infected intravenously with 10³ LD₅₀ of LCMV Traub. (A,B) On postinfection days 8, 10, 14, and 60, splenocytes were harvested and the frequency of LCMV-specific VLA-4⁺ IFN-γ ⁺ CD8⁺T cells (A) or CD4⁺ T cells (B) were evaluated by flow cytometry. (C) On day 0 (uninfected) and postinfection days 8, 10, and 14, spleens were harvested and the total number of cells per spleen was determined. The results are shown as histograms; columns represent group medians and bars represent ranges. Group sizes are shown below each column. The asterisk denotes P < .05, Mann-Whitney rank test versus wild-type mice.