Expansion of AGM-derived cells by coculture with FL cells.

Floating hematopoietic cells generated under various culture conditions were harvested after 10 days of incubation. GFP⁺ cells were counted by FACS. Under these culture conditions, 40% to 80% of floating cells were GFP⁺. Results are shown as fold expansion relative to the number of input cells. Note that FL cells provided a better microenvironment for blood cell expansion than did AGM cells. Data shown are the mean ± SD of at least triplicate experiments.