Characterization and in vivo effects of 4D7 trioma cells.

(A) Binding of immunoglobulin from trioma (▪, 4D7; ●, BiV) and the corresponding parental hybridoma supernatants (■, NLDC-145, ○, 2.4G2) to P388D₁ or S49 cells. Binding occurs by way of the rat moiety. Bound immunoglobulin is detected with fluorescein isothiocyanate-conjugated goat antirat IgG (parental hybridomas) or rat antimouse IgG (triomas). Maximum fluorescence is expressed as 100%. (B) Tumor protection conferred by 4D7 trioma cells. Animals were preimmunized and challenged as described in Figure 1. ▴, 4D7 vaccination; ×, tumor control.