RP105 does not interact with MD-2.

Ba/F3 cells expressing RP105 + MD-1 (lane 1), RP105 + MD-2 (lane 2), RP105/MD-1 (lane 3), or RP105/MD-1 + TLR4/MD-2 (lane 4) were used for immunoprecipitation with anti-RP105 mAb RP/14 (A) or with the anti-flag mAb M2 (B). RP105 was detected with cell surface biotinylation as described in “Materials and methods.” MD-1 (lanes 1, 3) and MD-2 (lanes 2, 4) were tagged with the Flag epitope and detected by immunoprobing with the anti-flag mAb.