Effect of an anti–MD-1 mAb on up-regulation of B7.2/CD86 and CD23.

Purified B cells were stimulated with lipid A (1.0 μg/mL), anti-RP105 (10 μg/mL), or anti-IgM (10 μg/mL) in the presence (lower panels) or absence (upper panels) of the anti–MD-1 mAb MD113 (10 μg/mL), as indicated. After 1 day, cells were harvested and stained with anti-B7.2 (A) or anti-CD23 (B). Open histograms (upper panels) and gray lines (lower panels) show cells stimulated with medium alone. Similar results were obtained from 3 independent experiments.