Fig. 2.
Fig. 2. Targeted disruption of the murine. / MD-1 gene by homologous recombination. (A) Schematic representation of the MD-1 gene, the targeting construct, and the targeted MD-1 locus. The first exon of theMD-1 gene is shown as a closed box. The indicated external probe detects 5.0 and 3.0 kb of the HindIII-digested genomic DNA of the wild-type and targeted MD-1 genes, respectively. (B) Southern blot analysis of the MD-1 mutation in mice. Tail DNA isolated from the wild-type, MD-1+/−, and MD-1–−/− mice was digested with HindIII, electrophoresed on an agarose gel, blotted onto nylon membrane, and hybridized with the probe, which is shown in panel A. (C) Spleen cells from wild-type (broken line) or MD-1−/− mice (thick line) were stained with an anti–MD-1 mAb MD14, followed by FITC-labeled goat anti–rat IgG.

Targeted disruption of the murine

MD-1 gene by homologous recombination. (A) Schematic representation of the MD-1 gene, the targeting construct, and the targeted MD-1 locus. The first exon of theMD-1 gene is shown as a closed box. The indicated external probe detects 5.0 and 3.0 kb of the HindIII-digested genomic DNA of the wild-type and targeted MD-1 genes, respectively. (B) Southern blot analysis of the MD-1 mutation in mice. Tail DNA isolated from the wild-type, MD-1+/−, and MD-1–−/− mice was digested with HindIII, electrophoresed on an agarose gel, blotted onto nylon membrane, and hybridized with the probe, which is shown in panel A. (C) Spleen cells from wild-type (broken line) or MD-1−/− mice (thick line) were stained with an anti–MD-1 mAb MD14, followed by FITC-labeled goat anti–rat IgG.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal