Establishment of anti–MD-1 mAbs.

The specificity of anti–MD-1 mAbs is shown with flow cytometry staining of Ba/F3 cells expressing either mouse RP105/mouse MD-1 (A, D, G), human RP105/mouse MD-1 (B, E, H), or human RP105/human MD-1 (C, F, I). These cells were stained with the mAbs to either mouse RP105, RP/14 (A-C), mouse MD-1, MD14 (D-F), or human RP105, MHR73 (G-I). FITC-labeled goat anti–rat IgG or anti–mouse IgG was used for the second reagent. Gray lines depict staining with the second antibody alone.