Immunohistology of CXCR4 and CXCR5.

Immunohistochemical detection on paraffin-embedded sections using the 2B11/CXCR4 (A-C) and 8B2/CXCR5 (E-G) monoclonal antibodies. Most follicular mantle cells and some germinal center cells express CXCR4 in a hyperplastic tonsil (A). The neoplastic cells of some follicular lymphomas (B) and a case of cHD (C) are strongly CXCR4 positive. (D) In situ hybridization reveals specific transcripts for SDF-1 in a case of cHD. (E-G) Immunohistology of CXCR5. Most cells in the mantle zone and some germinal center cells of a hyperplastic tonsil show CXCR5 expression (E). Some tumor cells of a cHD (G) and tumor cells of a follicular lymphoma (F) are positive. (H-J) In situ hybridization with a probe specific for BCA showed positive accessory cells in a germinal center of a hyperplastic tonsil (H). Reactive cells in cases of cHD (I) and NLPHD (J) are labeled, whereas the neoplastic cells are negative (arrows). Original magnification, × 20 (A), × 40 (B, E), × 100 (F), × 200 (C, D, G-J).