CCR7 expression is NF-κB dependent.

(A) NF-κB binding activity. Whole-cell extracts of HD cell lines and control cells were analyzed by EMSA using H2K binding site probe for NF-κB. (B) RNA was extracted 24 hours after infection from L428 cells infected with Ad5-control (L428c24) or Ad5-IκBΔN (L428i24), and from different control and HD cell lines. Northern blot analysis was performed using an α³²P-labeled CCR7 cDNA probe (upper panel). As a control, the stripped blot was reprobed with an α³²P-labeled GAPDH cDNA probe (lower panel).