Differential chemokine receptor RNA expression in HD-derived cell lines.

(A,B) RNase protection assay was performed using the hCR5 template set (A) containing DNA templates for CCR1, CCR2, CCR2a, CCR2b, CCR3, CCR4, CCR5, CCR8, GAPDH, and L32; and the hCR6 template (B) containing DNA templates for CXCR1, CXCR2, CXCR3, CXCR4, BLR1 (CXCR5), BLR2 (CCR7), V28, GAPDH, and L32. Seven HD cell lines were studied, along with an erythroid leukemia cell line as a negative control (Figure 2B). (C) Expression of the chemokine receptors CXCR5 and CCR7 in HD-derived cell lines. Northern blot analysis was performed using total RNA (20 μg) isolated from the HD cell lines L428, HD-MyZ, L1236, L540, HDLM-2, L591, and KM-H2. The blot was hybridized with CXCR5- and CCR7-specific cDNA probes and with GAPDH cDNA as a housekeeping probe.