Inhibition of cell growth by LMB-2 in combination with ODN.
(A) B-CLL cells were grown in medium supplemented with 1 μg/mL LMB-2 and indicated concentrations of ODN. After 5 days, cell viability was assessed with an MTS assay: 10 μL of a combined MTS/PMS solution were added to the cell suspension. The adsorbance at 490 nm was assessed with an ELISA reader. Results are expressed as percent OD490, related to the OD490 of cells grown without immunotoxin. Results represent mean plus or minus SEM of independent experiments with cells from 3 different patients. (B) Inhibition of cell growth was measured in a B-CLL sample at different time points in the presence of 1 μg/mL LMB-2 with or without DSP30. An independent experiment gave comparable results.
