Fig. 2.
Fig. 2. Developmental synchrony of erythroid progenitor cells from TAP-treated mice. / Mice were treated for 5 days with TAP to block the development of erythroid burst-forming units. TAP then was removed, erythropoiesis was allowed to advance, and at 72, 96, and 120 hours after TAP withdrawal, levels of c-Kit, EPO receptor and βmaj-globin transcripts in erythroid splenocytes were analyzed by Northern blotting (A). In addition, splenocytes isolated at 72 hours after TAP withdrawal were cultured in the presence of mSCF (150 ng/mL) and EPO (5 U/mL). At 24-hour intervals, expression of Ter119 antigen was assayed as an index of late differentiation (B). For each population, morphologies also were examined (insets, × 1000).

Developmental synchrony of erythroid progenitor cells from TAP-treated mice.

Mice were treated for 5 days with TAP to block the development of erythroid burst-forming units. TAP then was removed, erythropoiesis was allowed to advance, and at 72, 96, and 120 hours after TAP withdrawal, levels of c-Kit, EPO receptor and βmaj-globin transcripts in erythroid splenocytes were analyzed by Northern blotting (A). In addition, splenocytes isolated at 72 hours after TAP withdrawal were cultured in the presence of mSCF (150 ng/mL) and EPO (5 U/mL). At 24-hour intervals, expression of Ter119 antigen was assayed as an index of late differentiation (B). For each population, morphologies also were examined (insets, × 1000).

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