KKO antigenicity of recombinant PF4 mutant constructs by ELISA.

On the left of each figure are the constructs studied and their names. The right of each figure shows their antigenicity when complexed to heparin in an ELISA study with KKO. (A) The initial N-terminal switches are shown. (B) ELISA studies involving the switching of the major sequence difference between human and mouse PF4 in the second domain (Figure 1B, underlined) are shown. (C) Studies of switches between human and mouse PF4 and with NAP-2 and IL-8 are shown. Also shown are studies of point mutations at the N terminus of PF4 and at the Pro34 position. The names of the recombinant appear in gray for portions derived from human PF4 and in black for substitutions from other proteins.