Fig. 4.
Effects of As2O3 or cAMP or both on NB4-R1 cell proliferation.
(A) Cell viability was assessed by trypan blue dye exclusion method following the indicated treatments. Each value represented the mean ± SD of 3 independent measurements. (B) Flow cytometric analysis of cell cycle distribution by PI staining. One representative experiment among 3 independent assays is shown. (C) Western blot analysis of some cell cycle-involved proteins (E2F, p21, p27, cyclin D1, CDK4, and p53) expression level during the treatments.
