Fig. 3.
![Fig. 3. Size-exclusion FPLC analysis of nuclear RA-binding activity in COS-1 cells. / Cells are transfected with pSG5 vector (A), wild-type PML-RARα L form (B), mutant Lys207Asn (C), mutant Arg272Gln (D), mutant Gly289Arg (E), mutant Pro407Ser (F), wild-type PML-RARα S form (G), and mutant Arg294Trp (H). Nuclear extracts were incubated with 10 nmol/L [3H]-RA in the absence (●) or presence (○) of 200-fold excess of unlabeled RA for 15 hours at 4°C. The samples were fractionated over a Superose 6HR 10/30 column at 0.4-mL intervals. Arrows indicate the fraction numbers of marker proteins (in kd) used to calibrate the FPLC column.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/4/10.1182_blood.v99.4.1356/6/h80422151003.jpeg?Expires=1725021404&Signature=GasJefppIB0k3ABRh40O9CkbiUhGcUCex~Y8wdh2INXLLap5~jhjv2lk7ymi6fHgLDYg1J32lLjDDlJ3M6nQ6xxch7-jg68Eje8WfLVRGo-XYcB~-evVnIXYbyc80QN0HOohDRSU408d66o7q1fNzyvzkD73ioSg462AUIMOYrsLEbfwusFyrhBWsyp0BU7jo9J2qiUtOMQocBuuZUB5lTGqDnAmY2qQCtbHT1B8Z4bPoLmLuy4yVeDZJcDBDgHUD1NJGARrLDBxMOTk7w2pjli82NwA4QGTMfi-YlQo7kVTBqJZUkdnmflXISkxfU5-qn~8WlGsl2MsR9meVMdVWw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Size-exclusion FPLC analysis of nuclear RA-binding activity in COS-1 cells.
Cells are transfected with pSG5 vector (A), wild-type PML-RARα L form (B), mutant Lys207Asn (C), mutant Arg272Gln (D), mutant Gly289Arg (E), mutant Pro407Ser (F), wild-type PML-RARα S form (G), and mutant Arg294Trp (H). Nuclear extracts were incubated with 10 nmol/L [3H]-RA in the absence (●) or presence (○) of 200-fold excess of unlabeled RA for 15 hours at 4°C. The samples were fractionated over a Superose 6HR 10/30 column at 0.4-mL intervals. Arrows indicate the fraction numbers of marker proteins (in kd) used to calibrate the FPLC column.
