Effect of Hcy on cyclin A promoter activity in vascular cells.

HUVECs and RASMCs were transfected with 2 μg PGL2 plasmids containing SV40 early promoter or cyclin A promoter (−266/+205) by lipofectin transfection. Starting 24 hours after transfection, cells were treated with or without 50 μM dl-Hcy, and harvested 24 hours later. The corrected luciferase activity for each time point was divided by that of control (cyclin A −266/+205 plasmid in cells not treated with Hcy) and is presented as relative luciferase activity. Values represent the mean ± SD from 2 separate experiments (n = 6). *P < .01 versus control.