Suppression of cyclin A, but not cyclin D1 and cyclin E mRNA expression by Hcy.

HUVECs were exposed to 25 μM or 50 μM dl-Hcy for 30 hours (A), to 50 μM dl-Hcy or l-HH (B), and to 50 μM dl-Hcy or l-Cys (C) for the indicated times. Total cellular RNA (10 μg) was used for Northern blot analysis. The RNA blot was hybridized to human cyclin A, D1, and E probes successively, and then to an 18S oligonucleotide probe (n = 3).